Cell surface area staining was performed by incubating cells with anti-hCD4-V450 (eBioscience) or anti-hCD4-PECy7 (Beckton Dickinson) for 30 min at 4C at night. and was significant although humble, because just non-cognate MKp-T cell connections could be examined, under non-polarizing circumstances. Importantly, the MKp-mediated expansion was seen in the absence or presence of direct MKp-T cell … Continue reading Cell surface area staining was performed by incubating cells with anti-hCD4-V450 (eBioscience) or anti-hCD4-PECy7 (Beckton Dickinson) for 30 min at 4C at night